ELISA/assay

Human CD28 (Cluster of Differentiation 28) ELISA Kit

MBS2509748

24-Strip-Wells

215 USD

ELISA Kit

Human CD28 (Cluster of Differentiation 28) ELISA Kit

[CD28]

[CD28, partial; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28; CD28 antigen; CD_antigen: CD28]

[CD28]

[Cd28; Cd28]

CD28_MOUSE

Human

63

This kit recognizes natural and recombinantHumanCD28. No significant cross-reactivity or interference between HumanCD28 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.625-40ng/mL. Sensitivity: Min: 0.375ng/mL; Max: 40ng/mL

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanCD28concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CD28. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CD28and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CD28, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CD28.You can calculate the concentration of CD28in the samples by comparing the OD of the samples to the standard curve.

192485

AAA37396.1

P31041

25,243 Da

Adaptive Immune System Pathway (1110669); Allograft Rejection Pathway (83316); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (83314); Autoimmune Thyroid Disease Pathway (533); CD28 Co-stimulation Pathway (1110676); CD28 Dependent PI3K/Akt Signaling Pathway (1110677); CD28 Dependent Vav1 Pathway (1110678); Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480)

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described. Protein type: Membrane protein, integral; Cell surface. Cellular Component: membrane; plasma membrane; integral to membrane; immunological synapse; external side of plasma membrane; lipid raft. Molecular Function: protein homodimerization activity; protease binding; SH3/SH2 adaptor activity. Biological Process: positive regulation of isotype switching to IgG isotypes; T cell activation; positive regulation of mitosis; positive regulation of interleukin-2 biosynthetic process; negative thymic T cell selection; T cell receptor signaling pathway; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of inflammatory response to antigenic stimulus; positive regulation of protein kinase B signaling cascade; T cell costimulation; positive regulation of alpha-beta T cell proliferation; immune response; positive regulation of T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulatory T cell differentiation

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590