Human PLA2G10 (Phospholipase A2, Group X) ELISA Kit | MyBioSource MBS2509729 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human PLA2G10 (Phospholipase A2, Group X) ELISA Kit

catalog :

MBS2509729

quantity :

24-Strip-Wells

price :

215 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2509729

products type :

ELISA Kit

products full name :

Human PLA2G10 (Phospholipase A2, Group X) ELISA Kit

products short name :

[PLA2G10]

other names :

[PLA2G10; Group 10 secretory phospholipase A2; group 10 secretory phospholipase A2; GX sPLA2; group X secretory phospholipase A2; phosphatidylcholine 2-acylhydrolase 10; sPLA2-X; phospholipase A2, group X; Group X secretory phospholipase A2; GX sPLA2; sPLA2-X; Phosphatidylcholine 2-acylhydrolase 10]

products gene name :

[PLA2G10]

other gene names :

[PLA2G10; PLA2G10; SPLA2; GXPLA2; GXSPLA2; GX sPLA2; sPLA2-X]

uniprot entry name :

PA2GX_HUMAN

reactivity :

Human

sequence length :

165

specificity :

This kit recognizes Human PKM2 in samples. No significant cross-reactivity or interference between Human PKM2 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 78.13-5000pg/mL. Sensitivity: 46.88pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PKM2 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PKM2 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PKM2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PKM2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PKM2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PKM2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PKM2. You can calculate the concentration of Human PKM2 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

48145887

ncbi acc num :

CAG33166.1

uniprot acc num :

O15496

ncbi mol weight :

18,153 Da

ncbi pathways :

Acyl Chain Remodelling Of PC Pathway (645321); Acyl Chain Remodelling Of PE Pathway (645317); Acyl Chain Remodelling Of PG Pathway (645328); Acyl Chain Remodelling Of PI Pathway (645326); Acyl Chain Remodelling Of PS Pathway (645324); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Ether Lipid Metabolism Pathway (82990); Ether Lipid Metabolism Pathway (365); Fat Digestion And Absorption Pathway (194385)

uniprot summary :

PLA2G10: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine. Belongs to the phospholipase A2 family. Protein type: Phospholipase; Lipid Metabolism - arachidonic acid; Lipid Metabolism - ether lipid; Motility/polarity/chemotaxis; Lipid Metabolism - linoleic acid; Secreted, signal peptide; EC 3.1.1.4; Lipid Metabolism - glycerophospholipid; Secreted; Lipid Metabolism - alpha-linolenic acid; Cell development/differentiation. Chromosomal Location of Human Ortholog: 16p13.1-p12. Cellular Component: extracellular region. Molecular Function: phospholipase A2 activity; calcium ion binding; phospholipase activity. Biological Process: axon guidance; cholesterol homeostasis; negative regulation of transcription factor activity; regulation of macrophage activation; phospholipid metabolic process; lysophospholipid transport; positive regulation of cellular protein metabolic process; positive regulation of prostaglandin secretion; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; arachidonic acid metabolic process; lipid catabolic process

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590

more info or order :

MyBioSource product webpage