catalog number :
MBS2509694
products type :
ELISA Kit
products full name :
Human LMP7/PSMB8 (Low molecular mass protein 7/Proteasome subunit beta type-8) ELISA Kit
products short name :
[LMP7/PSMB8 (Low molecular mass protein 7/Proteasome subunit beta type-8)]
products gene name :
[LMP7/PSMB8]
specificity :
This kit recognizes Human LL-37 in samples. No significant cross-reactivity or interference between Human LL-37 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: 0.94ng/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human LL-37 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human LL-37 were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human LL-37 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LL-37. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LL-37 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LL-37, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human LL-37. You can calculate the concentration of Human LL-37 in the samples by comparing the OD of the samples to the standard curve.
size5 :
10x96-Strip-Wells