ELISA/assay

Human 5-HETE (5-Hydroxyeicosatetraenoic Acid) ELISA Kit

MBS2509624

48-Strip-Wells

410 USD

ELISA Kit

Human 5-HETE (5-Hydroxyeicosatetraenoic Acid) ELISA Kit

5-HETE

5-HETE

Human

This kit recognizes natural and recombinant Human 5-HETE. No significant cross-reactivity or interference between Human 5-HETE and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Competitive. Detection Range: 0.156-10ng/mL. Sensitivity: 0.094ng/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human 5-HETE concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 5-HETE. During the reaction, 5-HETE in the sample or standard competes with a fixed amount of 5-HETE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 5-HETE. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of 5-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.

48-Strip-Wells

410 USD

96-Strip-Wells

490