ELISA/assay

Rat ADAMTS13/VWFCP(Von Willebrand Factor Cleaving Protease)ELISA Kit

MBS2509576

48-Strip-Wells

410 USD

ELISA Kit

Rat ADAMTS13/VWFCP(Von Willebrand Factor Cleaving Protease)ELISA Kit

ADAMTS13/VWFCP

A disintegrin and metalloproteinase with thrombospondin motifs 13 isoform 2 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 13; A disintegrin and metalloproteinase with thrombospondin motifs 13; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13; vWF-cleaving protease; von Willebrand factor-cleaving protease; ADAM metallopeptidase with thrombospondin type 1 motif, 13; von Willebrand factor-cleaving protease; vWF-CP; vWF-cleaving protease

ADAMTS13/VWFCP

ADAMTS13; ADAMTS13; VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13; C9orf8; ADAM-TS 13; ADAM-TS13; ADAMTS-13; vWF-CP; vWF-cleaving protease

ATS13_HUMAN

Rat

1371

This assay has high sensitivity and excellent specificity for detection of Rat ADAMTS13/VWFCP. No significant cross-reactivity or interference between Rat ADAMTS13/VWFCP and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat ADAMTS13/VWFCP and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 78.125--5000pg/mL. Sensitivity: 46.875pg/mL

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ADAMTS13/VWFCP in Rat serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ADAMTS13/VWFCP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat ADAMTS13/VWFCP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ADAMTS13/VWFCP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat ADAMTS13/VWFCP. You can calculate the concentration of Rat ADAMTS13/VWFCP in the samples by comparing the OD of the samples to the standard curve.

73695936

NP_620596.2

NM_139027.4

Q76LX8

39,864 Da

Metabolism Of Proteins Pathway (106230); O-glycosylation Of TSR Domain-containing Proteins Pathway (1127680); O-linked Glycosylation Pathway (1127679); Post-translational Protein Modification Pathway (161012)

This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

ADAMTS13: Cleaves the vWF multimers in plasma into smaller forms. Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP); also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.24.87; Extracellular matrix; Motility/polarity/chemotaxis; Secreted, signal peptide; Calcium-binding; Protease; Secreted. Chromosomal Location of Human Ortholog: 9q34. Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; endoplasmic reticulum lumen. Molecular Function: integrin binding; protein binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding. Biological Process: integrin-mediated signaling pathway; protein amino acid O-linked glycosylation; platelet activation; cellular protein metabolic process; glycoprotein metabolic process; cell-matrix adhesion; peptide catabolic process; response to toxin; protein processing; post-translational protein modification; proteolysis. Disease: Thrombotic Thrombocytopenic Purpura, Congenital

48-Strip-Wells

410 USD

96-Strip-Wells

490