catalog number :
MBS2509464
products type :
ELISA Kit
products full name :
Human ApoA2 (Apolipoprotein A2) ELISA Kit
products short name :
[ApoA2]
other names :
[apolipoprotein A-II preproprotein; Apolipoprotein A-II; apolipoprotein A-II; apolipoprotein A2; apolipoprotein A-II; Apolipoprotein A2Cleaved into the following 2 chains:Proapolipoprotein A-II; ProapoA-II; Truncated apolipoprotein A-IIAlternative name(s):Apolipoprotein A-II(1-76)]
products gene name :
[ApoA2]
other gene names :
[APOA2; APOA2; apoAII; Apo-AII; ApoA-II; Apo-AII; ApoA-II; ProapoA-II]
uniprot entry name :
APOA2_HUMAN
specificity :
This kit recognizes Human ApoA1 in samples. No significant cross-reactivity or interference between HumanApoA1and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 3.13-200ng/mL. Sensitivity: 1.88ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level HumanApoA1were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level HumanApoA1were testedon 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human ApoA1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successivelyto each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ApoA1. You can calculate the concentration of Human ApoA1 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001634.1
ncbi gb acc num :
NM_001643.1
ncbi mol weight :
11,175 Da
ncbi pathways :
Chylomicron-mediated Lipid Transport Pathway (106157); Complement And Coagulation Cascades Pathway (198880); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPAR Signaling Pathway (83042)
ncbi summary :
This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia. [provided by RefSeq, Jul 2008]
uniprot summary :
APOA2: May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. Belongs to the apolipoprotein A2 family. Protein type: Secreted; Secreted, signal peptide; Endoplasmic reticulum; Lipid-binding. Chromosomal Location of Human Ortholog: 1q23.3. Cellular Component: chylomicron; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol. Molecular Function: lipid transporter activity; protein binding; protein homodimerization activity; lipase inhibitor activity; protein heterodimerization activity; cholesterol transporter activity; cholesterol binding; phospholipid binding; phosphatidylcholine binding; lipid binding; high-density lipoprotein binding; apolipoprotein receptor binding. Biological Process: positive regulation of catalytic activity; phototransduction, visible light; protein folding; viral reproduction; negative regulation of cholesterol transport; response to glucocorticoid stimulus; regulation of protein stability; diacylglycerol catabolic process; positive regulation of lipid catabolic process; cellular lipid metabolic process; negative regulation of lipase activity; negative regulation of lipid catabolic process; regulation of cholesterol absorption; phospholipid efflux; response to glucose stimulus; retinoid metabolic process; response to drug; cholesterol metabolic process; organ regeneration; protein amino acid oxidation; phospholipid catabolic process; positive regulation of interleukin-8 biosynthetic process; lipoprotein metabolic process; cholesterol efflux; negative regulation of cytokine secretion during immune response; cholesterol homeostasis; triacylglycerol metabolic process; reverse cholesterol transport; response to estrogen stimulus; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; acute inflammatory response. Disease: Hypercholesterolemia, Familial
size5 :
10x96-Strip-Wells