catalog number :
MBS2509454
products type :
ELISA Kit
products full name :
Porcine IL-6 (Interleukin 6) ELISA Kit
products short name :
[IL-6]
other names :
[interleukin-6; Interleukin-6; interleukin-6; IL-6; Interleukin 6 (interferon, beta 2); interleukin 6]
products gene name :
[IL-6]
other gene names :
[Il6; Il6; ILg6; Ifnb2; Il-6; IL-6]
uniprot entry name :
IL6_RAT
specificity :
This kit recognizes natural and recombinantPorcineIL-6. No significant cross-reactivity or interference between PorcineIL-6 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 125-8000pg/mL. Sensitivity: Min: 75pg/mL; Max: 8000pg/mL
products description :
Intended Uses: This ELISA kit applies to the invitro quantitative determination of PorcineIL-6concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-6. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-6and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-6.You can calculate the concentration of IL-6in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_036721.1
ncbi gb acc num :
NM_012589.2
ncbi mol weight :
24,357 Da
ncbi pathways :
ARMS-mediated Activation Pathway (1120656); Activated TLR4 Signalling Pathway (1121566); Adipogenesis Pathway (198479); African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Amoebiasis Pathway (167311); Amoebiasis Pathway (167191); Androgen Receptor Signaling Pathway (198529); Axon Guidance Pathway (1121351); Cellular Senescence Pathway (1121344)
ncbi summary :
a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]
uniprot summary :
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation ().
size5 :
10x96-Strip-Wells