Human PLAP/ALPP(Placental Alkaline Phosphatase)ELISA Kit | MyBioSource MBS2509358 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human PLAP/ALPP(Placental Alkaline Phosphatase)ELISA Kit

catalog :

MBS2509358

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2509358

products type :

ELISA Kit

products full name :

Human PLAP/ALPP(Placental Alkaline Phosphatase)ELISA Kit

products short name :

PLAP/ALPP

other names :

PREDICTED: alkaline phosphatase, placental type isoform X1; Alkaline phosphatase, placental type; alkaline phosphatase, placental type; alkaline phosphatase Regan isozyme; alkaline phosphomonoesterase; glycerophosphatase; placental alkaline phosphatase 1; alkaline phosphatase, placental; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1

products gene name :

PLAP/ALPP

other gene names :

ALPP; ALPP; ALP; PALP; PLAP; PLAP-1; PLAP; PLAP-1

uniprot entry name :

PPB1_HUMAN

reactivity :

Human

sequence length :

550

specificity :

This assay has high sensitivity and excellent specificity for detection of Human PLAP/ALPP. No significant cross-reactivity or interference between Human PLAP/ALPP and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PLAP/ALPP and all the analogues, therefore, cross reaction may still exist.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156--10ng/mL. Sensitivity: 0.094ng/mL

products description :

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PLAP/ALPP in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PLAP/ALPP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PLAP/ALPP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLAP/ALPP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PLAP/ALPP. You can calculate the concentration of Human PLAP/ALPP in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

530370113

ncbi acc num :

XP_005246496.1

ncbi gb acc num :

XM_005246439.2

uniprot acc num :

P05187

ncbi mol weight :

57,954 Da

ncbi pathways :

Folate Biosynthesis Pathway (83018); Folate Biosynthesis Pathway (404); Metabolic Pathways (132956); Pyridoxal 5'-phosphate Salvage Pathway (142237)

ncbi summary :

The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]

uniprot summary :

ALPP: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3 untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]. Protein type: Cell surface; Membrane protein, integral; Motility/polarity/chemotaxis; Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - folate biosynthesis; EC 3.1.3.1; Phosphatase (non-protein). Chromosomal Location of Human Ortholog: 2q37. Cellular Component: cell surface; integral to membrane; plasma membrane. Molecular Function: alkaline phosphatase activity; zinc ion binding; magnesium ion binding. Biological Process: dephosphorylation

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage