ELISA/assay

Rat NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

MBS2509356

24-Strip-Wells

215 USD

ELISA Kit

Rat NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

[NT-proBNP]

[natriuretic peptides A preproprotein; Natriuretic peptides A; natriuretic peptides A; atriopeptin; cardionatrin; natriuretic peptide precursor A; prepronatriodilatin; natriuretic peptide A; CDD-ANF; Cardiodilatin; CDD; Cardiodilatin-related peptide; CDP; PrepronatriodilatinAtrial natriuretic factor; ANFAlternative name(s):Atrial natriuretic peptide; ANP]

[NT-proBNP]

[NPPA; NPPA; ANF; ANP; PND; ATFB6; ATRST2; CDD-ANF; ANP; PND; CDD; CDP; ANF; ANP]

ANF_HUMAN

Rat

151

This kit recognizes Rat NT-ProANP in samples. No Significant cross-reactivity or interference between Rat NT-ProANP and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.31-20 ng/mL. Sensitivity: 0.19 ng/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat NT-ProANP were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat NT-ProANP were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat NT-ProANP concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NT-ProANP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NT-ProANP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NT-ProANP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat NT-ProANP. You can calculate the concentration of Rat NT-ProANP in the samples by comparing the OD of the samples to the standard curve.

23510319

NP_006163.1

NM_006172.3

P01160

16,708 Da

Amyloids Pathway (366238); Disease Pathway (530764); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); HIF-1 Signaling Pathway (695200); MicroRNAs In Cardiomyocyte Hypertrophy Pathway (198784); YAP1- And WWTR1 (TAZ)-stimulated Gene Expression Pathway (576276)

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq, Sep 2009]

NPPA: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Defects in NPPA are the cause of familial atrial fibrillation type 6 (ATFB6). Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity, progressive deterioration of atrial electromechanical function and ineffective pumping of blood into the ventricles. It can be associated with palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the natriuretic peptide family. Protein type: Secreted; Hormone; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1p36.21. Cellular Component: extracellular space; mast cell granule; perinuclear region of cytoplasm; extracellular region; nucleus. Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; receptor binding. Biological Process: cardiac muscle hypertrophy; transcription initiation from RNA polymerase II promoter; cGMP biosynthetic process; negative regulation of systemic arterial blood pressure; positive regulation of heart rate; female pregnancy; response to insulin stimulus; neuropeptide signaling pathway; regulation of blood pressure; receptor guanylyl cyclase signaling pathway; response to hypoxia; gene expression; negative regulation of cell growth; regulation of blood vessel size. Disease: Atrial Standstill 2; Atrial Fibrillation, Familial, 6

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590