ELISA/assay

Rat CASP3 (Caspase 3) ELISA Kit

MBS2509349

24-Strip-Wells

215 USD

ELISA Kit

Rat CASP3 (Caspase 3) ELISA Kit

[CASP3]

[caspase-3 preproprotein; Caspase-3; caspase-3; CASP-3; CPP-32; PARP cleavage protease; SREBP cleavage activity 1; apopain; caspase 3, apoptosis-related cysteine protease; cysteine protease CPP32; procaspase3; protein Yama; caspase 3, apoptosis-related cysteine peptidase; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1]

[CASP3]

[CASP3; CASP3; CPP32; SCA-1; CPP32B; CPP32; CASP-3; CPP-32; SCA-1]

CASP3_HUMAN

Rat

277

This kit recognizes natural and recombinantRatCASP3. No significant cross-reactivity or interference between RatCASP3 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL

Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatCASP3concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CASP3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CASP3and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CASP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CASP3.You can calculate the concentration of CASP3in the samples by comparing the OD of the samples to the standard curve.

14790119

NP_004337.2

NM_004346.3

P42574

31,608 Da

AGE/RAGE Pathway (698754); Activation Of DNA Fragmentation Factor Pathway (105683); Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway (105672); Alpha6-Beta4 Integrin Signaling Pathway (198807); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975)

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a 216-Asp- -Gly-217 bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family. Protein type: Apoptosis; EC 3.4.22.56; Protease; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 4q34. Cellular Component: nucleoplasm; plasma membrane; nucleus; cytosol. Molecular Function: cyclin-dependent protein kinase inhibitor activity; peptidase activity; protein binding; cysteine-type endopeptidase activity; aspartic-type endopeptidase activity. Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; negative regulation of activated T cell proliferation; heart development; negative regulation of B cell proliferation; regulation of caspase activity; proteolysis; neuron differentiation; extracellular matrix disassembly; sensory perception of sound; B cell homeostasis; positive regulation of neuron apoptosis; response to wounding; erythrocyte differentiation; T cell homeostasis; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis; response to UV; release of cytochrome c from mitochondria; cell fate commitment; negative regulation of cyclin-dependent protein kinase activity; keratinocyte differentiation; neuron apoptosis; induction of apoptosis via death domain receptors; platelet formation; caspase activation via cytochrome c; response to DNA damage stimulus; induction of apoptosis by oxidative stress; negative regulation of apoptosis

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590