catalog number :
MBS2509345
products type :
ELISA Kit
products full name :
Human HSPbeta6/HSP-20 (Heat Shock Protein Beta 6) ELISA Kit
products short name :
[HSPbeta6/HSP-20]
other names :
[hsp 20 family small heat shock protein; hsp 20 family small heat shock protein]
products gene name :
[HSPbeta6/HSP-20]
other gene names :
[SSO2603]
specificity :
This kit recognizes natural and recombinant Human HSPbeta6/HSP-20. No significant cross-reactivity or interference between Human HSPbeta6/HSP-20 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human HSPbeta6/HSP-20 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HSPbeta6/HSP-20. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HSPbeta6/HSP-20 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HSPbeta6/HSP-20, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HSPbeta6/HSP-20. You can calculate the concentration of HSPbeta6/HSP-20 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_343935.1
ncbi gb acc num :
NC_002754.1
size5 :
10x96-Strip-Wells