Human C2 (Complement Component 2) ELISA Kit | MyBioSource MBS2509340 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human C2 (Complement Component 2) ELISA Kit

catalog :

MBS2509340

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2509340

products type :

ELISA Kit

products full name :

Human C2 (Complement Component 2) ELISA Kit

products short name :

[C2]

other names :

[C2, partial; Complement C2; complement C2; C3/C5 convertase; complement component C2; complement component 2; C3/C5 convertaseCleaved into the following 2 chains:Complement C2b fragment; Complement C2a fragment]

products gene name :

[C2]

other gene names :

[C2; C2; CO2; ARMD14]

uniprot entry name :

CO2_HUMAN

reactivity :

Human

sequence length :

577

specificity :

This kit recognizes natural and recombinantHumanC2. No significant cross-reactivity or interference between HumanC2 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.625-40ng/mL. Sensitivity: Min: 0.375ng/mL; Max: 40ng/mL

products description :

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanC2concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to C2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for C2and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain C2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of C2.You can calculate the concentration of C2in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

15277207

uniprot acc num :

P06681

ncbi mol weight :

69,444 Da

ncbi pathways :

Activation Of C3 And C5 Pathway (106412); Complement Activation, Classical Pathway (198823); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Immune System Pathway (106386); Initial Triggering Of Complement Pathway (106406); Innate Immune System Pathway (106387); Pertussis Pathway (218111)

ncbi summary :

Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined.[provided by RefSeq, Mar 2009]

uniprot summary :

C2: Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase. Defects in C2 are the cause of complement component 2 deficiency (C2D). A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Protease; EC 3.4.21.43; Secreted. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: extracellular space; extracellular region. Molecular Function: serine-type endopeptidase activity; metal ion binding. Biological Process: regulation of complement activation; innate immune response; proteolysis; complement activation, classical pathway; response to nutrient; complement activation. Disease: Macular Degeneration, Age-related, 14; Complement Component 2 Deficiency

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590