catalog number :
MBS2509314
products type :
ELISA Kit
products full name :
Rat ALP (Alkaline Phosphatase) ELISA Kit
products short name :
[ALP]
other names :
[antileukoproteinase; Antileukoproteinase; antileukoproteinase; ALP; secretory leukocyte protease inhibitor; secretory leukocyte peptidase inhibitor; Secretory leukocyte protease inhibitor]
products gene name :
[ALP]
other gene names :
[Slpi; Slpi; ALP]
uniprot entry name :
SLPI_MOUSE
specificity :
This kit recognizes Rat ACTH in samples. No significant cross-reactivity or interference between Rat ACTH and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat ACTH were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat ACTH were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat ACTH concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat ACTH. During the reaction, Rat ACTH in the sample or standard competes with a fixed amount of Rat ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat ACTH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Rat ACTH in the samples is then determined by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_035544.1
ncbi gb acc num :
NM_011414.3
ncbi mol weight :
14,308 Da
uniprot summary :
SLPI: Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues. Protein type: Secreted, signal peptide; Secreted; Inhibitor. Cellular Component: extracellular matrix; extracellular region. Molecular Function: serine-type endopeptidase inhibitor activity; enzyme binding; protease inhibitor activity; endopeptidase inhibitor activity. Biological Process: negative regulation of viral genome replication; negative regulation of peptidase activity; negative regulation of protein binding
size5 :
10x96-Strip-Wells