ELISA/assay

Mouse ACTH (Adrencocorticotropic Hormone) ELISA Kit

MBS2509224

96-Strip-Wells

445 USD

ELISA Kit

Mouse ACTH (Adrencocorticotropic Hormone) ELISA Kit

ACTH

ACTH, partial

ACTH

Mouse

41

This kit recognizes natural and recombinant Mouse ACTH. No significant cross-reactivity or interference between Mouse ACTH and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Quantitative Competitive. Sensitivity: 9.375pg/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse ACTH concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with ACTH. During the reaction, ACTH in the sample or standard competes with a fixed amount of ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ACTH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ACTH in the samples is then determined by comparing the OD of the samples to the standard curve.

490074

CAA00890.1

96-Strip-Wells

445 USD