ELISA/assay

Human GRIK2 (Glutamate Receptor, Ionotropic, Kainate 2) ELISA Kit

MBS2509191

24-Strip-Wells

215 USD

ELISA Kit

Human GRIK2 (Glutamate Receptor, Ionotropic, Kainate 2) ELISA Kit

[GRIK2]

[glutamate receptor ionotropic, kainate 2 isoform 2; Glutamate receptor ionotropic, kainate 2; glutamate receptor ionotropic, kainate 2; bA487F5.1; excitatory amino acid receptor 4; gluR-6; glutamate receptor 6; glutamate receptor form A; glutamate receptor form B; glutamate receptor form C; glutamate receptor form D; glutamate receptor form E; glutamate receptor, ionotropic, kainate 2; Excitatory amino acid receptor 4; EAA4; Glutamate receptor 6; GluR-6; GluR6]

[GRIK2]

[GRIK2; GRIK2; EAA4; GLR6; MRT6; GLUK6; GLUR6; GluK2; GLUR6; GluK2; EAA4; GluR-6; GluR6]

GRIK2_HUMAN

Human

869

This kit recognizes natural and recombinant Human GRIK2. No significant cross-reactivity or interference between Human GRIK2 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human GRIK2 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GRIK2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GRIK2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GRIK2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GRIK2. You can calculate the concentration of GRIK2 in the samples by comparing the OD of the samples to the standard curve.

28559003

NP_786944.1

NM_175768.3

Q13002

77,112 Da

Activation Of Ca-permeable Kainate Receptor Pathway (161046); Activation Of Kainate Receptors Upon Glutamate Binding Pathway (161043); Activation Of Na-permeable Kainate Receptors Pathway (161045); Glutamatergic Synapse Pathway (213818); Glutamatergic Synapse Pathway (213811); Ionotropic Activity Of Kainate Receptors Pathway (161044); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Neuronal System Pathway (106513); Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway (106534)

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing at multiple sites within the first and second transmembrane domains, which is thought to alter the structure and function of the receptor complex. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. Mutations in this gene have been associated with autosomal recessive mental retardation. [provided by RefSeq, Jul 2008]

GluR6: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Modulates cell surface expression of NETO2. Defects in GRIK2 are the cause of mental retardation autosomal recessive type 6 (MRT6). It is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic mental retardation. MRT6 patients display mild to severe mental retardation and psychomotor development delay in early childhood. Patients do not have neurologic problems, congenital malformations, or facial dysmorphism. Body height, weight, and head circumference are normal. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK2 subfamily. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Channel, ligand-gated; Channel, calcium; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 6q16.3. Cellular Component: dendrite cytoplasm; presynaptic membrane; postsynaptic membrane; kainate selective glutamate receptor complex; integral to plasma membrane; postsynaptic density; dendrite; plasma membrane; terminal button; perikaryon; cell junction. Molecular Function: protein homodimerization activity; kainate selective glutamate receptor activity; extracellular-glutamate-gated ion channel activity; ubiquitin conjugating enzyme binding; ubiquitin protein ligase binding; PDZ domain binding. Biological Process: regulation of synaptic transmission; regulation of long-term neuronal synaptic plasticity; synaptic transmission, glutamatergic; behavioral fear response; glutamate signaling pathway; generation of action potential; negative regulation of synaptic transmission, glutamatergic; regulation of short-term neuronal synaptic plasticity; receptor clustering; regulation of JNK cascade; positive regulation of synaptic transmission; cellular calcium ion homeostasis; synaptic transmission; intracellular protein transport; regulation of inhibitory postsynaptic membrane potential; neuron apoptosis; transport; ionotropic glutamate receptor signaling pathway; positive regulation of neuron apoptosis; negative regulation of neuron apoptosis; regulation of excitatory postsynaptic membrane potential. Disease: Mental Retardation, Autosomal Recessive 6

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590