catalog number :
MBS2509174
products type :
ELISA Kit
products full name :
Rat MDC/CCL22 (Macrophage-Derived Chemokine) ELISA Kit
products short name :
[MDC/CCL22]
other names :
[macrophage-derived chemokine CCL22; C-C motif chemokine; C-C motif chemokine 22; small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys) member 22; small inducible cytokine subfamily A (Cys-Cys), member 22; chemokine (C-C motif) ligand 22]
products gene name :
[MDC/CCL22]
other gene names :
[Ccl22; MDC; Mdc; Scya22]
uniprot entry name :
Q91ZH5_RAT
specificity :
This kit recognizes Rat LH in samples. No significant cross-reactivity or interference between Rat LH and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.56-100mIU/mL. Sensitivity: 0.94mIU/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat LH were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat LH were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat LH concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat LH. You can calculate the concentration of Rat LH in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAL30397.2
ncbi mol weight :
10,391 Da
ncbi pathways :
Chemokine Receptors Bind Chemokines Pathway (1120716); Chemokine Signaling Pathway (98755); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (1120714); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1122016); Disease Pathway (1121849); GPCR Ligand Binding Pathway (1120713); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1121989)
ncbi summary :
chemoattractant for dendritic cells, natural killer (NK) cells, and the Th2 subset of peripheral blood T cells [RGD, Feb 2006]
size5 :
10x96-Strip-Wells