ELISA/assay

Human MUC2 (Mucin 2) ELISA Kit

MBS2509166

48-Strip-Wells

410 USD

ELISA Kit

Human MUC2 (Mucin 2) ELISA Kit

MUC2

mucin-2; Mucin-2; mucin-2; intestinal mucin-2; mucin 2, intestinal/tracheal; mucin-like protein; mucin 2, oligomeric mucus/gel-forming; Intestinal mucin-2

MUC2

MUC2; MUC2; MLP; SMUC; MUC-2; SMUC; MUC-2

MUC2_HUMAN

Human

5179

This kit recognizes natural and recombinant Human MUC2. No significant cross-reactivity or interference between Human MUC2 and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: 0.188ng/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human MUC2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MUC2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MUC2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MUC2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of MUC2. You can calculate the concentration of MUC2 in the samples by comparing the OD of the samples to the standard curve.

557129045

NP_002448.3

NM_002457.3

Q02817

540,300 Da

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Pathway (1127679); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Termination Of O-glycan Biosynthesis Pathway (530748); Vibrio Cholerae Infection Pathway (83101); Vibrio Cholerae Infection Pathway (513)

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008]

MUC2: Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. Protein type: Secreted, signal peptide; Secreted; Extracellular matrix. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: Golgi lumen. Molecular Function: protein binding. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; maintenance of gastrointestinal epithelium; post-translational protein modification

48-Strip-Wells

410 USD

96-Strip-Wells

490