Human IDE (Insulin Degrading Enzyme) ELISA Kit | MyBioSource MBS2509139 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human IDE (Insulin Degrading Enzyme) ELISA Kit

catalog :

MBS2509139

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2509139

products type :

ELISA Kit

products full name :

Human IDE (Insulin Degrading Enzyme) ELISA Kit

products short name :

IDE

other names :

insulin-degrading enzyme isoform 2; Insulin-degrading enzyme; insulin-degrading enzyme; Abeta-degrading protease; insulin protease; insulinase; insulin-degrading enzyme; Abeta-degrading protease; Insulin protease; Insulinase; Insulysin

products gene name :

IDE

other gene names :

IDE; IDE; INSULYSIN; Insulinase

uniprot entry name :

IDE_HUMAN

reactivity :

Human

sequence length :

464

specificity :

This assay has high sensitivity and excellent specificity for detection of Human IDE. No significant cross-reactivity or interference between Human IDE and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human IDE and all the analogues, therefore, cross reaction may still exist.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 62.5--4000pg/mL. Sensitivity: 37.5pg/mL

products description :

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IDE in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IDE. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human IDE and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IDE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human IDE. You can calculate the concentration of Human IDE in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

260099676

ncbi acc num :

NP_001159418.1

ncbi gb acc num :

NM_001165946.1

uniprot acc num :

P14735

ncbi mol weight :

54,240 Da

ncbi pathways :

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448)

ncbi summary :

This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009]

uniprot summary :

IDE: Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. Belongs to the peptidase M16 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Protease; EC 3.4.24.56; Nuclear receptor co-regulator; Cell surface. Chromosomal Location of Human Ortholog: 10q23-q25. Cellular Component: nucleoplasm; peroxisomal matrix; extracellular space; cell surface; mitochondrion; cytoplasm; plasma membrane; peroxisome; cytosol; nucleus. Molecular Function: ubiquitin binding; protein homodimerization activity; zinc ion binding; beta-amyloid binding; metalloendopeptidase activity; ATPase activity; peptide binding; beta-endorphin binding; insulin binding; protein binding; glycoprotein binding; ATP binding; receptor binding. Biological Process: negative regulation of proteolysis; positive regulation of protein oligomerization; proteolysis involved in cellular protein catabolic process; viral reproduction; protein heterooligomerization; beta-amyloid metabolic process; insulin receptor signaling pathway; hormone catabolic process; determination of adult life span; proteolysis; protein homooligomerization; protein homotetramerization

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage