Human MIF (Macrophage Migration Inhibitory Factor) ELISA Kit | MyBioSource MBS2508982 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human MIF (Macrophage Migration Inhibitory Factor) ELISA Kit

catalog :

MBS2508982

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2508982

products type :

ELISA Kit

products full name :

Human MIF (Macrophage Migration Inhibitory Factor) ELISA Kit

products short name :

[MIF]

other names :

[MIF; Macrophage migration inhibitory factor; macrophage migration inhibitory factor; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase]

products gene name :

[MIF]

other gene names :

[MIF; MIF; GIF; GLIF; MMIF; GLIF; MMIF; MIF; GIF]

uniprot entry name :

MIF_HUMAN

reactivity :

Human

sequence length :

115

specificity :

This assay has high sensitivity and excellent specificity for detection of Human MIF. No significant cross-reactivity or interference between Human MIF and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human MIF and all the analogues, therefore, cross reaction may still exist.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.781-50ng/mL. Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL

products description :

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MIF in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MIF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human MIF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human MIF. You can calculate the concentration of Human MIF in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

47678571

ncbi acc num :

CAG30406.1

uniprot acc num :

P14174

ncbi mol weight :

12,476 Da

ncbi pathways :

Adipogenesis Pathway (198832); Phenylalanine Metabolism Pathway (82960); Phenylalanine Metabolism Pathway (327); Spinal Cord Injury Pathway (739007); Tyrosine Metabolism Pathway (82959); Tyrosine Metabolism Pathway (325)

ncbi summary :

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]

uniprot summary :

MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase. Protein type: EC 5.3.3.12; Amino Acid Metabolism - phenylalanine; Cytokine; EC 5.3.2.1; Isomerase; Amino Acid Metabolism - tyrosine; Cell development/differentiation; Apoptosis. Chromosomal Location of Human Ortholog: 22q11.23. Cellular Component: nucleoplasm; extracellular space; cell surface; cytoplasm; extracellular region; vesicle. Molecular Function: phenylpyruvate tautomerase activity; protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity; dopachrome isomerase activity; receptor binding; chemoattractant activity. Biological Process: DNA damage response, signal transduction by p53 class mediator; regulation of macrophage activation; carboxylic acid metabolic process; positive regulation of lipopolysaccharide-mediated signaling pathway; cell aging; negative regulation of cellular protein metabolic process; prostaglandin biosynthetic process; positive regulation of peptidyl-serine phosphorylation; cell proliferation; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of MAP kinase activity; negative regulation of myeloid cell apoptosis; positive regulation of fibroblast proliferation; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; negative regulation of mature B cell apoptosis; positive chemotaxis; positive regulation of B cell proliferation; innate immune response; inflammatory response; positive regulation of phosphorylation; negative regulation of apoptosis; positive regulation of cytokine secretion. Disease: Rheumatoid Arthritis, Systemic Juvenile

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590