ELISA/assay

Mouse ICAM-3(intercellular adhesion molecule 3)ELISA Kit

MBS2508947

48-Strip-Wells

410 USD

ELISA Kit

Mouse ICAM-3(intercellular adhesion molecule 3)ELISA Kit

ICAM-3

intercellular adhesion molecule 3; Intercellular adhesion molecule 3; intercellular adhesion molecule 3; ICAM-3; intercellular adhesion molecule-3; intercellular adhesion molecule 3; CDw50; ICAM-R; CD_antigen: CD50

ICAM-3/CD50

ICAM3; ICAM3; CD50; CDW50; ICAM-R; ICAM-3

ICAM3_HUMAN

Mouse

547

This assay has high sensitivity and excellent specificity for detection of Mouse ICAM-3/CD50. No significant cross-reactivity or interference between Mouse ICAM-3/CD50 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse ICAM-3/CD50 and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.391--25ng/mL. Sensitivity: 0.234ng/mL

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ICAM-3/CD50 in Mouse serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ICAM-3/CD50. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse ICAM-3/CD50 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ICAM-3/CD50, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse ICAM-3/CD50. You can calculate the concentration of Mouse ICAM-3/CD50 in the samples by comparing the OD of the samples to the standard curve.

167466207

NP_002153.2

NM_002162.3

P32942

59,541 Da

Adaptive Immune System Pathway (366160); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Extracellular Matrix Organization Pathway (576262); IL-5 Signaling Pathway (198770); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413); Integrin Cell Surface Interactions Pathway (106110)

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. [provided by RefSeq, Jul 2008]

ICAM3: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. Belongs to the immunoglobulin superfamily. ICAM family. Protein type: Membrane protein, integral; Cell adhesion; Immunoglobulin superfamily. Chromosomal Location of Human Ortholog: 19p13.3-p13.2. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: integrin binding; protein binding; receptor binding. Biological Process: extracellular matrix organization and biogenesis; regulation of immune response; cell-cell adhesion; cell adhesion

48-Strip-Wells

410 USD

96-Strip-Wells

490