catalog number :
MBS2508923
products type :
ELISA Kit
products full name :
Mouse SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit
products short name :
[SPD]
other names :
[Spd; Spd; Spd; Spd]
products gene name :
[SP-D]
other gene names :
[pWTY27_6; pWTY27_6]
uniprot entry name :
D2KKW1_9ACTO
specificity :
This kit recognizes natural and recombinant Mouse SP-D. No significant cross-reactivity or interference between Mouse SP-D and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse SP-D concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SP-D. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SP-D and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SP-D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SP-D. You can calculate the concentration of SP-D in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
YP_003358133.1
ncbi gb acc num :
NC_013667.2
ncbi mol weight :
40,924 Da
size5 :
10x96-Strip-Wells