Rabbit HGF (Hepatocyte Growth Factor) ELISA Kit | MyBioSource MBS2508913 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rabbit HGF (Hepatocyte Growth Factor) ELISA Kit

catalog :

MBS2508913

quantity :

24-Strip-Wells

price :

235 USD

product information

catalog number :

MBS2508913

products type :

ELISA Kit

products full name :

Rabbit HGF (Hepatocyte Growth Factor) ELISA Kit

products short name :

[HGF]

other names :

[hepatocyte growth factor isoform 1 preproprotein; Hepatocyte growth factor; hepatocyte growth factor; fibroblast-derived tumor cytotoxic factor; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoietin-A; Scatter factor; SF]

products gene name :

[HGF]

other gene names :

[HGF; HGF; SF; HGFB; HPTA; F-TCF; DFNB39; HPTA; SF]

uniprot entry name :

HGF_HUMAN

reactivity :

Rabbit

sequence length :

728

specificity :

This kit recognizes natural and recombinant Rabbit HGF. No significant cross-reactivity or interference between Rabbit HGF and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit HGF concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HGF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HGF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HGF. You can calculate the concentration of HGF in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

33859835

ncbi acc num :

NP_000592.3

ncbi gb acc num :

NM_000601.4

uniprot acc num :

P14210

ncbi mol weight :

24,116 Da

ncbi pathways :

Arf6 Signaling Events Pathway (138034); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Direct P53 Effectors Pathway (137939); FGF Signaling Pathway (137989); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Hemostasis Pathway (106028)

ncbi summary :

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

HGF: HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity. Activating ligand for the receptor tyrosine kinase MET by binding and promoting its dimerization. Defects in HGF are the cause of deafness autosomal recessive type 39 (DFNB39). A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the peptidase S1 family. Plasminogen subfamily. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Hormone; Cell development/differentiation. Chromosomal Location of Human Ortholog: 7q21.1. Cellular Component: extracellular space; membrane; extracellular region. Molecular Function: identical protein binding; protein binding; growth factor activity; protein heterodimerization activity; serine-type endopeptidase activity; chemoattractant activity. Biological Process: positive regulation of myelination; platelet activation; mitosis; organ regeneration; activation of MAPK activity; myoblast proliferation; hepatocyte growth factor receptor signaling pathway; negative regulation of caspase activity; proteolysis; liver development; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of osteoblast differentiation; positive regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; platelet degranulation; positive chemotaxis; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; hyaluronan metabolic process; positive regulation of cell migration. Disease: Deafness, Autosomal Recessive 39

size1 :

24-Strip-Wells

price1 :

235 USD

size2 :

48-Strip-Wells

price2 :

450

size3 :

96-Strip-Wells

price3 :

535

size4 :

5x96-Strip-Wells

price4 :

2255

size5 :

10x96-Strip-Wells

price5 :

3975