Human EPOR (Erythropoietin Receptor) ELISA Kit | MyBioSource MBS2508879 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human EPOR (Erythropoietin Receptor) ELISA Kit

catalog :

MBS2508879

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2508879

products type :

ELISA Kit

products full name :

Human EPOR (Erythropoietin Receptor) ELISA Kit

products short name :

[EPOR]

other names :

[erythropoietin receptor; Erythropoietin receptor; erythropoietin receptor; erythropoietin receptor]

products gene name :

[EPOR]

other gene names :

[EPOR; EPOR; EPO-R; EPO-R]

uniprot entry name :

EPOR_HUMAN

reactivity :

Human

sequence length :

508

specificity :

This kit recognizes natural and recombinant Human EPOR. No significant cross-reactivity or interference between Human EPOR and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human EPOR concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to EPOR. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for EPOR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain EPOR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of EPOR. You can calculate the concentration of EPOR in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

4503591

ncbi acc num :

NP_000112.1

ncbi gb acc num :

NM_000121.3

uniprot acc num :

P19235

ncbi mol weight :

35,809 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); EPO Receptor Signaling Pathway (198882); EPO Signaling Pathway (138051); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Kit Receptor Signaling Pathway (198826); PI3K-Akt Signaling Pathway (692234)

ncbi summary :

This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

uniprot summary :

EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling. Protein type: Membrane protein, integral; Receptor, cytokine. Chromosomal Location of Human Ortholog: 19p13.3-p13.2. Cellular Component: integral to plasma membrane; extracellular region. Molecular Function: identical protein binding; protein binding; erythropoietin receptor activity. Biological Process: heart development; decidualization; brain development; signal transduction. Disease: Erythrocytosis, Familial, 1

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590