catalog number :
MBS2508855
products type :
ELISA Kit
products full name :
Rabbit IL-4 (Interleukin 4) ELISA Kit
products short name :
IL-4
other names :
interleukin-4; Interleukin-4; interleukin-4; B-cell stimulatory factor 1; BSF-1; IL-4; lymphocyte stimulatory factor 1; B-cell stimulatory factor 1; BSF-1; Lymphocyte stimulatory factor 1
products gene name :
IL-4
other gene names :
IL4; IL4; IL-4; BSF-1
uniprot entry name :
IL4_PIG
specificity :
This kit recognizes natural and recombinant Rabbit IL-4. No significant cross-reactivity or interference between Rabbit IL-4 and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: The minimum detectable dose of Rabbit IL-4 is 9.375pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit IL-4 concentrations in serum, plasma and other biological fluids.
products description :
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-4. You can calculate the concentration of IL-4 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_999288.1
ncbi gb acc num :
NM_214123.1
ncbi mol weight :
15,023 Da
ncbi pathways :
Allograft Rejection Pathway (84530); Allograft Rejection Pathway (535); Asthma Pathway (84527); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (84528); Autoimmune Thyroid Disease Pathway (533); Cytokine-cytokine Receptor Interaction Pathway (84463); Cytokine-cytokine Receptor Interaction Pathway (460); Fc Epsilon RI Signaling Pathway (84494); Fc Epsilon RI Signaling Pathway (493)