Monkey PON1 (Paraoxonase 1) ELISA Kit | MyBioSource MBS2508850 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey PON1 (Paraoxonase 1) ELISA Kit

catalog :

MBS2508850

quantity :

24-Strip-Wells

price :

235 USD

product information

catalog number :

MBS2508850

products type :

ELISA Kit

products full name :

Monkey PON1 (Paraoxonase 1) ELISA Kit

products short name :

[PON1]

other names :

[paraoxonase; Serum paraoxonase/arylesterase 1; serum paraoxonase/arylesterase 1; A-esterase 1; K-45; PON 1; aromatic esterase 1; arylesterase B-type; esterase A; paraoxonase B-type; serum aryldiakylphosphatase; serum aryldialkylphosphatase 1; paraoxonase 1; Aromatic esterase 1; A-esterase 1; K-45; Serum aryldialkylphosphatase 1]

products gene name :

[PON1]

other gene names :

[PON1; PON1; ESA; PON; MVCD5; PON; PON 1; A-esterase 1]

uniprot entry name :

PON1_HUMAN

reactivity :

Monkey

sequence length :

355

specificity :

This kit recognizes Monkey PIIINP in samples. No Significant cross-reactivity or interference between MonkeyPIIINP and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 23.44-1500 pg/mL. Sensitivity: 14.06pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level MonkeyPIIINP were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level MonkeyPIIINP were tested on 3 different plates, 20replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey PIIINP concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PIIINP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PIIINP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PIIINP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/-2 nm. The OD value is proportional to the concentration of PIIINP. You can calculate the concentration of PIIINP in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

1575043

ncbi acc num :

AAB41835.1

uniprot acc num :

P27169

ncbi mol weight :

39,731 Da

ncbi pathways :

Metabolic Pathways (132956); Phase I, Non P450 Pathway (198854)

ncbi summary :

The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq, Oct 2008]

uniprot summary :

PON1: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients. Belongs to the paraoxonase family. Protein type: Secreted, signal peptide; EC 3.1.1.2; Hydrolase; EC 3.1.8.1; Motility/polarity/chemotaxis; Secreted; EC 3.1.1.81; Phosphatase (non-protein); Lipid-binding. Chromosomal Location of Human Ortholog: 7q21.3. Cellular Component: extracellular space; intracellular membrane-bound organelle; extracellular region. Molecular Function: protein homodimerization activity; arylesterase activity; phospholipid binding; calcium ion binding; aryldialkylphosphatase activity. Biological Process: response to external stimulus; response to nutrient levels; dephosphorylation; organophosphate catabolic process; response to toxin; positive regulation of transporter activity; carboxylic acid catabolic process; positive regulation of binding; aromatic compound catabolic process; phosphatidylcholine metabolic process. Disease: Microvascular Complications Of Diabetes, Susceptibility To, 5

size1 :

24-Strip-Wells

price1 :

235 USD

size2 :

48-Strip-Wells

price2 :

450

size3 :

96-Strip-Wells

price3 :

535

size4 :

5x96-Strip-Wells

price4 :

2255

size5 :

10x96-Strip-Wells

price5 :

3975