catalog number :
MBS2508830
products type :
ELISA Kit
products full name :
Rat CRP (C-Reactive Protein) ELISA Kit
products short name :
[CRP]
other names :
[cAMP-activated global transcriptional regulator CRP; DNA-binding transcriptional dual regulator]
products gene name :
[CRP]
specificity :
This kit recognizes Rat CRH in samples. No significant cross-reactivity or interference between Rat CRH and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 0.31-20ng/mL. Sensitivity: 0.19ng/mL.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat CRH concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat CRH. During the reaction, Rat CRH in the sample or standard competes with a fixed amount of Rat CRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat CRH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Rat CRH in the samples is then determined by comparing the OD of the samples to the standard curve.
ncbi pathways :
Two-component System Pathway (6845); Two-component System Pathway (437)
ncbi summary :
[More information is available at EcoCyc: EG10164].
size5 :
10x96-Strip-Wells