ELISA/assay

Human HLA-B27 (Human Leukocyte Antigen B27) ELISA Kit

MBS2508747

24-Strip-Wells

215 USD

ELISA Kit

Human HLA-B27 (Human Leukocyte Antigen B27) ELISA Kit

[HLA-B27]

[HLA-B27, partial; HLA class I histocompatibility antigen, B-27 alpha chain; MHC class I antigen B*27]

[HLA-B27]

[HLA-B; HLAB]

1B27_HUMAN

Human

362

This kit recognizes natural and recombinantHumanHLA-B27. No significant cross-reactivity or interference between HumanHLA-B27 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanHLA-B27concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HLA-B27. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HLA-B27and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HLA-B27, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HLA-B27.You can calculate the concentration of HLA-B27in the samples by comparing the OD of the samples to the standard curve.

32177

CAA27578.1

P03989

40,428 Da

HLAB iso7: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family. Protein type: Membrane protein, integral; Immunoglobulin superfamily. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; integral to plasma membrane; early endosome membrane; endoplasmic reticulum; plasma membrane; integral to membrane; MHC class I protein complex. Molecular Function: peptide antigen binding; chaperone binding; TAP binding; receptor binding. Biological Process: regulation of immune response; viral reproduction; detection of bacterium; regulation of interleukin-12 production; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; defense response; protection from natural killer cell mediated cytotoxicity; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; regulation of T cell anergy; regulation of interleukin-6 production; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; immune response

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590