Mouse IL-1alpha(Interleukin 1 Alpha)ELISA Kit | MyBioSource MBS2508700 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse IL-1alpha(Interleukin 1 Alpha)ELISA Kit

catalog :

MBS2508700

quantity :

96-Strip-Wells

price :

445 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2508700

products type :

ELISA Kit

products full name :

Mouse IL-1alpha(Interleukin 1 Alpha)ELISA Kit

products short name :

IL-1alpha

other names :

interleukin-1 alpha proprotein; Interleukin-1 alpha; interleukin-1 alpha; IL-1 alpha; hematopoietin-1; preinterleukin 1 alpha; pro-interleukin-1-alpha; interleukin 1, alpha; Hematopoietin-1

products gene name :

IL-1alpha

other gene names :

IL1A; IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA; IL1F1; IL-1 alpha

uniprot entry name :

IL1A_HUMAN

reactivity :

Mouse

sequence length :

271

specificity :

This assay has high sensitivity and excellent specificity for detection of Mouse IL-1alpha. No significant cross-reactivity or interference between Mouse IL-1alpha and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse IL-1alpha and all the analogues, therefore, cross reaction may still exist.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625--1000pg/mL. Sensitivity: 9.375pg/mL

products description :

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL-1alpha in Mouse serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1alpha. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1alpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-1alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse IL-1alpha. You can calculate the concentration of Mouse IL-1alpha in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

27894330

ncbi acc num :

NP_000566.3

ncbi gb acc num :

NM_000575.3

uniprot acc num :

P01583

ncbi mol weight :

30,607 Da

ncbi pathways :

Apoptosis Pathway (83060); Apoptosis Pathway (470); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); FAS Pathway And Stress Induction Of HSP Regulation (198894); Graft-versus-host Disease Pathway (83124)

ncbi summary :

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

uniprot summary :

IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family. Protein type: Motility/polarity/chemotaxis; Cytokine. Chromosomal Location of Human Ortholog: 2q14. Cellular Component: extracellular space; extracellular region; cytosol. Molecular Function: protein binding; copper ion binding; interleukin-1 receptor binding; cytokine activity. Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; apoptosis; cytokine and chemokine mediated signaling pathway; positive regulation of interleukin-2 biosynthetic process; germ cell programmed cell death; positive regulation of JNK cascade; fever; cell proliferation; negative regulation of cell proliferation; connective tissue replacement during inflammatory response; positive regulation of angiogenesis; response to copper ion; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response; positive regulation of cytokine secretion

size1 :

96-Strip-Wells

price1 :

445 USD

more info or order :

MyBioSource product webpage