ELISA/assay

Human COL1alpha1 (Collagen Type I Alpha 1) ELISA Kit

MBS2508669

48-Strip-Wells

410 USD

ELISA Kit

Human COL1alpha1 (Collagen Type I Alpha 1) ELISA Kit

COL1alpha1

collagen alpha-1(II) chain; Collagen alpha-1(II) chain; collagen alpha-1(II) chain; collagen alpha-1(II) chain; Procollagen II alpha 1; alpha-1 type II collagen; collagen type II (Col2A1) gene, enhancer region; procollagen, type II, alpha 1; collagen, type II, alpha 1; Alpha-1 type II collagenCleaved into the following 2 chains:Collagen alpha-1(II) chain; Chondrocalcin

COL1alpha1

Col2a1; Col2a1; CG2A1A; COLLII

CO2A1_RAT

Human

1419

This assay has high sensitivity and excellent specificity for detection of Human COL1alpha1. No significant cross-reactivity or interference between Human COL1alpha1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human COL1alpha1 and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313--20ng/mL. Sensitivity: 0.188ng/mL

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL1alpha1 in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL1alpha1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human COL1alpha1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL1alpha1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human COL1alpha1. You can calculate the concentration of Human COL1alpha1 in the samples by comparing the OD of the samples to the standard curve.

6978677

NP_037061.1

NM_012929.1

P05539

134,570 Da

Amoebiasis Pathway (167311); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1121484); Collagen Biosynthesis And Modifying Enzymes Pathway (1121483); Collagen Formation Pathway (1121482); ECM-receptor Interaction Pathway (83460); ECM-receptor Interaction Pathway (479); Endochondral Ossification Pathway (219771); Extracellular Matrix Organization Pathway (1121481); Focal Adhesion Pathway (83459)

structural component of the cartilage extracellular matrix [RGD, Feb 2006]

48-Strip-Wells

410 USD

96-Strip-Wells

490