catalog number :
MBS2508639
products type :
ELISA Kit
products full name :
Mouse HSP gp96 (Heat Shock Protein glycoprotein 96) ELISA Kit
products short name :
[HSP gp96]
products gene name :
[HSP gp96]
specificity :
This kit recognizes natural and recombinant Mouse HSP gp96. No significant cross-reactivity or interference between Mouse HSP gp96 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 1.563-100ng/mL. Sensitivity: Min: 0.938ng/mL; Max: 100ng/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse HSP gp96 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HSP gp96. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HSP gp96 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HSP gp96, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HSP gp96. You can calculate the concentration of HSP gp96 in the samples by comparing the OD of the samples to the standard curve.
size5 :
10x96-Strip-Wells