catalog number :
MBS2508590
products type :
ELISA Kit
products full name :
Rat TAT (Thrombin-Antithrombin Complex) ELISA Kit
products short name :
[TAT]
other names :
[Tat; p14; Tat]
products gene name :
[TAT]
specificity :
This kit recognizes natural and recombinant Rat TAT. No significant cross-reactivity or interference between Rat TAT and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat TAT concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TAT. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TAT and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TAT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TAT. You can calculate the concentration of TAT in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_057853.1
ncbi gb acc num :
NC_001802.1
ncbi pathways :
Disease Pathway (530764); Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway (106004); HIV Infection Pathway (105983); HIV Life Cycle Pathway (105984); HIV Transcription Elongation Pathway (106001); Host Interactions Of HIV Factors Pathway (106010); Interactions Of Tat With Host Cellular Proteins Pathway (106018); Late Phase Of HIV Life Cycle Pathway (105995); Pausing And Recovery Of Tat-mediated HIV Elongation Pathway (161055); Signaling Events Mediated By HDAC Class III Pathway (137972)
size5 :
10x96-Strip-Wells