Human BMP-1 (Bone Morphogenetic Protein 1) ELISA Kit | MyBioSource MBS2508469 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human BMP-1 (Bone Morphogenetic Protein 1) ELISA Kit

catalog :

MBS2508469

quantity :

96-Strip-Wells

price :

445 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2508469

products type :

ELISA Kit

products full name :

Human BMP-1 (Bone Morphogenetic Protein 1) ELISA Kit

products short name :

BMP-1

other names :

bone morphogenetic protein, partial; Bone morphogenetic protein 1; bone morphogenetic protein 1; mammalian tolloid protein; procollagen C-endopeptidase; procollagen C-proteinase 3; bone morphogenetic protein 1; Mammalian tolloid protein; mTld; Procollagen C-proteinase; PCP

products gene name :

BMP-1

other gene names :

BMP1; BMP1; PCP; TLD; OI13; PCP2; PCOLC; PCOLC; BMP-1; mTld; PCP

uniprot entry name :

BMP1_HUMAN

reactivity :

Human

sequence length :

284

specificity :

This assay has high sensitivity and excellent specificity for detection of Human BMP-1. No significant cross-reactivity or interference between Human BMP-1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human BMP-1 and all the analogues, therefore, cross reaction may still exist.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156--10ng/mL. Sensitivity: 0.094ng/mL

products description :

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BMP-1 in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BMP-1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human BMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human BMP-1. You can calculate the concentration of Human BMP-1 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

619861

ncbi acc num :

AAC41710.1

uniprot acc num :

P13497

ncbi mol weight :

92,655 Da

ncbi pathways :

Adipogenesis Pathway (198832); Anchoring Fibril Formation Pathway (730307); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Cardiac Progenitor Differentiation Pathway (712094); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Crosslinking Of Collagen Fibrils Pathway (730308); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); HDL-mediated Lipid Transport Pathway (106158)

ncbi summary :

This gene encodes a protein that is capable of inducing formation of cartilage in vivo. Although other bone morphogenetic proteins are members of the TGF-beta superfamily, this gene encodes a protein that is not closely related to other known growth factors. This gene is expressed as alternatively spliced variants that share an N-terminal protease domain but differ in their C-terminal region. [provided by RefSeq, Aug 2008]

uniprot summary :

BMP1: Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD). Defects in BMP1 are a cause of autosomal recessive osteogenesis imperfecta (AR-OI). A connective tissue disorder characterized by bone fragility, progressively deforming bones, bowing of limbs due to multiple fractures, very short stature, a triangular face, severe scoliosis, and grayish sclera. AR-OI due to BMP1 mutations belongs to the group of osteogenesis imperfecta type III in the Sillence classification. Belongs to the peptidase M12A family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.24.19; Cytokine; Protease. Chromosomal Location of Human Ortholog: 8p21.3. Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; extracellular region. Molecular Function: peptidase activity; growth factor activity; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; cytokine activity; calcium ion binding. Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; ossification; multicellular organismal development; lipoprotein metabolic process; proteolysis; cell differentiation; skeletal development; cartilage condensation. Disease: Osteogenesis Imperfecta, Type Xiii

size1 :

96-Strip-Wells

price1 :

445 USD

more info or order :

MyBioSource product webpage