Human IL-12 (Interleukin 12) ELISA Kit | MyBioSource MBS2508400 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human IL-12 (Interleukin 12) ELISA Kit

catalog :

MBS2508400

quantity :

24-Strip-Wells

price :

190 USD

product information

catalog number :

MBS2508400

products type :

ELISA Kit

products full name :

Human IL-12 (Interleukin 12) ELISA Kit

products short name :

[IL-12]

other names :

[Il-12 receptor beta2; Interleukin-12 receptor subunit beta-2; interleukin-12 receptor subunit beta-2; IL-12 receptor subunit beta-2; IL-12R subunit beta-2; interleukin-12 receptor beta-2 chain; interleukin 12 receptor, beta 2]

products gene name :

[IL-12]

other gene names :

[IL12RB2; IL12RB2; IL-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12R-beta-2; IL-12RB2]

uniprot entry name :

I12R2_HUMAN

reactivity :

Human

sequence length :

862

specificity :

This kit recognizes Human IL-10 in samples. No significant cross-reactivity or interference `between Human IL-10 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 7.81-500pg/mL. Sensitivity: 4.69pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-10 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-10 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human IL-10 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human IL-10. You can calculate the concentration of Human IL-10 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

1685028

ncbi acc num :

AAB36675.1

uniprot acc num :

Q99665

ncbi mol weight :

87,484 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL12-mediated Signaling Events Pathway (137922); IL27-mediated Signaling Events Pathway (137929); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488)

ncbi summary :

The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. Several transcript variants encoding different isoforms and non-protein coding transcripts have been found for this gene. [provided by RefSeq, Apr 2012]

uniprot summary :

IL12RB2: Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production. Belongs to the type I cytokine receptor family. Type 2 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, cytokine; Membrane protein, integral; Cell cycle regulation. Chromosomal Location of Human Ortholog: 1p31.3-p31.2. Cellular Component: integral to plasma membrane; external side of plasma membrane. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein kinase binding. Biological Process: positive regulation of interferon-gamma production; cell surface receptor linked signal transduction; peptidyl-tyrosine phosphorylation; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; interferon-gamma production; response to lipopolysaccharide

size1 :

24-Strip-Wells

price1 :

190 USD

size2 :

48-Strip-Wells

price2 :

310

size3 :

96-Strip-Wells

price3 :

355

size4 :

5x96-Strip-Wells

price4 :

1435

size5 :

10x96-Strip-Wells

price5 :

2805