Human PRG4 (Proteoglycan 4) ELISA Kit | MyBioSource MBS2508393 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human PRG4 (Proteoglycan 4) ELISA Kit

catalog :

MBS2508393

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2508393

products type :

ELISA Kit

products full name :

Human PRG4 (Proteoglycan 4) ELISA Kit

products short name :

[PRG4]

other names :

[proteoglycan 4 isoform A; Proteoglycan 4; proteoglycan 4; articular superficial zone protein; hemangiopoietin; lubricin; megakaryocyte stimulating factor; superficial zone proteoglycan; proteoglycan 4; Lubricin; Megakaryocyte-stimulating factor; Superficial zone proteoglycanProteoglycan 4 C-terminal part]

products gene name :

[PRG4]

other gene names :

[PRG4; PRG4; MSF; SZP; CACP; HAPO; JCAP; MSF; SZP]

uniprot entry name :

PRG4_HUMAN

reactivity :

Human

sequence length :

1404

specificity :

This kit recognizes Human PRDX5 in samples. No significant cross-reactivity or interference between HumanPRDX5and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: 0.1ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level HumanPRDX5were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level HumanPRDX5were testedon 3 different plates, 20 replicates in eachplate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PRDX5 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PRDX5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PRDX5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRDX5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PRDX5. You can calculate the concentration of Human PRDX5 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

67190163

ncbi acc num :

NP_005798.2

ncbi gb acc num :

NM_005807.3

uniprot acc num :

Q92954

ncbi mol weight :

146,468 Da

ncbi summary :

The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

uniprot summary :

PRG4: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface. Defects in PRG4 are the cause of camptodactyly- arthropathy-coxa vara-pericarditis syndrome (CACP); also known as Jacobs syndrome. CACP is an autosomal recessive disorder. Individuals with CACP have normal appearing joints at birth but with advancing age develop joint failure associated with noninflammatory synoviocyte hyperplasia and subintimal fibrosis of the synovial capsule. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q25-q31. Cellular Component: extracellular space. Molecular Function: scavenger receptor activity; polysaccharide binding. Biological Process: cell proliferation; receptor-mediated endocytosis; negative regulation of interleukin-6 biosynthetic process; immune response; regulation of cell proliferation. Disease: Camptodactyly-arthropathy-coxa Vara-pericarditis Syndrome

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590