Mouse RUNX2 (Runt Related Transcription Factor 2) ELISA Kit | MyBioSource MBS2508302 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse RUNX2 (Runt Related Transcription Factor 2) ELISA Kit

catalog :

MBS2508302

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2508302

products type :

ELISA Kit

products full name :

Mouse RUNX2 (Runt Related Transcription Factor 2) ELISA Kit

products short name :

[RUNX2]

other names :

[RUNX2 protein; Runt-related transcription factor 2; runt-related transcription factor 2; PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit; acute myeloid leukemia 3 protein; core-binding factor, runt domain, alpha subunit 1; oncogene AML-3; osteoblast-specific transcription factor 2; polyomavirus enhancer-binding protein 2 alpha A subunit; runt-related transcription factor 2; Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; CBF-alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; OSF-2; Polyomavirus enhancer-binding protein 2 alpha A subunit; PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit]

products gene name :

[RUNX2]

other gene names :

[RUNX2; RUNX2; CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1; AML3; CBFA1; OSF2; PEBP2A; CBF-alpha-1; OSF-2; PEA2-alpha A; PEBP2-alpha A]

uniprot entry name :

RUNX2_HUMAN

reactivity :

Mouse

sequence length :

172

specificity :

This kit recognizes Mouse PVRL2 in samples. No significant cross-reactivity or interference between Mouse PVRL2 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 78.13-5000pg/mL. Sensitivity: 46.88pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse PVRL2 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse PVRL2 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse PVRL2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PVRL2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PVRL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PVRL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse PVRL2. You can calculate the concentration of Mouse PVRL2 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

80477928

ncbi acc num :

AAI08920.1

uniprot acc num :

Q13950

ncbi mol weight :

54,249 Da

ncbi pathways :

Androgen Receptor Signaling Pathway (198806); BMP Signalling And Regulation Pathway (198910); Endochondral Ossification Pathway (198812); FGF Signaling Pathway (137989); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Interleukin-11 Signaling Pathway (698753); Notch-mediated HES/HEY Network Pathway (169347); Regulation Of Nuclear SMAD2/3 Signaling Pathway (137963); Regulation Of Retinoblastoma Protein Pathway (137916)

ncbi summary :

This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2008]

uniprot summary :

AML3: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5 - PYGPYGGT-3 , of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation. Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors. Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 and HIPK3. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with KAT6A and KAT6B. Binds to cyclin B1 CCNB1. Interacts with DDX5. Specifically expressed in osteoblasts. 3 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 6p21. Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm. Molecular Function: protein domain specific binding; protein binding; bHLH transcription factor binding; chromatin binding; transcription factor activity; ATP binding. Biological Process: embryonic forelimb morphogenesis; transcription initiation from RNA polymerase II promoter; ossification; positive regulation of transcription, DNA-dependent; cell maturation; regulation of fibroblast growth factor receptor signaling pathway; chondrocyte development; embryonic cranial skeleton morphogenesis; stem cell differentiation; odontogenesis of dentine-containing teeth; osteoblast development; BMP signaling pathway; osteoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of chondrocyte differentiation; negative regulation of smoothened signaling pathway; positive regulation of cell proliferation; gene expression; negative regulation of transcription, DNA-dependent; osteoblast fate commitment; regulation of odontogenesis of dentine-containing teeth; T cell differentiation; endochondral ossification. Disease: Metaphyseal Dysplasia With Maxillary Hypoplasia With Or Without Brachydactyly; Cleidocranial Dysplasia

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590