catalog number :
MBS2508277
products type :
ELISA Kit
products full name :
Mouse PLA2G2D (Phospholipase A2, Group IID) ELISA Kit
products short name :
[PLA2G2D]
other names :
[PLA2G2D; Phospholipase A(2)]
products gene name :
[PLA2G2D]
other gene names :
[UNQ768]
uniprot entry name :
Q6UWX0_HUMAN
specificity :
This kit recognizes natural and recombinant Mouse PLA2G2D. No significant cross-reactivity or interference between Mouse PLA2G2D and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse PLA2G2D concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PLA2G2D. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PLA2G2D and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PLA2G2D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PLA2G2D. You can calculate the concentration of PLA2G2D in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAQ88969.1
ncbi mol weight :
12,910 Da
size5 :
10x96-Strip-Wells