ELISA/assay

Porcine LZM (Lysozyme) ELISA Kit

MBS2508195

48-Strip-Wells

450 USD

ELISA Kit

Porcine LZM (Lysozyme) ELISA Kit

LZM

lysozyme C; Lysozyme C; lysozyme C; 1,4-beta-N-acetylmuramidase C; c-type lysozyme; lysozyme; 1,4-beta-N-acetylmuramidase C

LZM

LYZ; LYZ; LZM; LZM

LYSC_HUMAN

Porcine

148

This kit recognizes natural and recombinant Porcine LZM. No significant cross-reactivity or interference between Porcine LZM and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: The minimum detectable dose of Porcine LZM is 0.1ng/mL (The sensitivity of this assay, or lowest detectable limit(LDL) was defined as the lowest protein concentration that could be differentiated from zero).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine LZM concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LZM. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LZM and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LZM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of LZM.You can calculate the concentration of LZMin the samples by comparing the O.D. of the samples to the standard curve.

4557894

NP_000230.1

NM_000239.2

P61626

16,537 Da

Amyloids Pathway (366238); C-MYB Transcription Factor Network Pathway (138073); Disease Pathway (530764); Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352)

This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. [provided by RefSeq, Oct 2014]

LYZ: Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte- macrophage system and enhance the activity of immunoagents. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8); also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. Belongs to the glycosyl hydrolase 22 family. Protein type: Secreted, signal peptide; Endoplasmic reticulum; Hydrolase; Vesicle; Secreted; EC 3.2.1.17. Chromosomal Location of Human Ortholog: 12q15. Cellular Component: extracellular space; extracellular region. Molecular Function: identical protein binding; lysozyme activity. Biological Process: retinal homeostasis; metabolic process; cytolysis; defense response to bacterium; inflammatory response. Disease: Amyloidosis, Familial Visceral

48-Strip-Wells

450 USD

96-Strip-Wells

535