Sheep ANG2 (Angiopoietin 2) ELISA Kit | MyBioSource MBS2508132 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Sheep ANG2 (Angiopoietin 2) ELISA Kit

catalog :

MBS2508132

quantity :

48-Strip-Wells

price :

490 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2508132

products type :

ELISA Kit

products full name :

Sheep ANG2 (Angiopoietin 2) ELISA Kit

products short name :

ANG2

other names :

angiopoietin-2 isoform c; Angiopoietin-2; angiopoietin-2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a; angiopoietin 2

products gene name :

ANG2

other gene names :

ANGPT2; ANGPT2; ANG2; AGPT2; ANG-2

uniprot entry name :

ANGP2_HUMAN

reactivity :

Sheep

sequence length :

444

specificity :

This kit recognizes natural and recombinant Sheep ANG2. No significant cross-reactivity or interference between Sheep ANG2 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of Sheep ANG2 is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep ANG2 concentrations in serum, plasma and other biological fluids.

products description :

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ANG2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ANG2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ANG2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ANG2. You can calculate the concentration of ANG2 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

169646754

ncbi acc num :

NP_001112360.1

ncbi gb acc num :

NM_001118888.1

uniprot acc num :

O15123

ncbi mol weight :

56,848 Da

ncbi pathways :

Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Cell Surface Interactions At The Vascular Wall Pathway (106062); HIF-1 Signaling Pathway (695200); Hemostasis Pathway (106028); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Rap1 Signaling Pathway (868086); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (868085); Tie2 Signaling Pathway (106063)

ncbi summary :

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

ANGPT2: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 8p23.1. Cellular Component: extracellular space; cell projection; plasma membrane; extracellular region; nucleus. Molecular Function: protein binding; metal ion binding; receptor tyrosine kinase binding; receptor binding. Biological Process: organ regeneration; Tie receptor signaling pathway; maternal process involved in pregnancy; negative regulation of blood vessel endothelial cell migration; signal transduction; response to organic cyclic substance; germ cell development; negative regulation of positive chemotaxis; response to radiation; positive regulation of angiogenesis; negative regulation of angiogenesis; response to mechanical stimulus; response to hypoxia; response to glucose stimulus; response to activity; angiogenesis; blood coagulation; leukocyte migration

size1 :

48-Strip-Wells

price1 :

490 USD

size2 :

96-Strip-Wells

price2 :

585

more info or order :

MyBioSource product webpage