ELISA/assay

Human TNX (Tenascin X) ELISA Kit

MBS2508101

48-Strip-Wells

410 USD

ELISA Kit

Human TNX (Tenascin X) ELISA Kit

TNX

tenascin-X isoform 1; Tenascin-X; tenascin-X; growth-inhibiting protein 45; hexabrachion-like protein; tenascin XB1; tenascin XB2; tenascin XB; Hexabrachion-like protein

TNX

TNXB; TNXB; XB; TNX; XBS; EDS3; HXBL; TENX; TN-X; VUR8; TNXB1; TNXB2; TNXBS; HXBL; TNX; TNXB1; TNXB2; XB; TN-X

TENX_HUMAN

Human

4242

This assay has high sensitivity and excellent specificity for detection of Human TNX. No significant cross-reactivity or interference between Human TNX and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human TNX and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 1.563--100ng/mL. Sensitivity: 0.938ng/mL

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNX in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNX. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human TNX and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNX, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human TNX. You can calculate the concentration of Human TNX in the samples by comparing the OD of the samples to the standard curve.

188528648

NP_061978.6

NM_019105.6

P22105

458,220 Da

ECM Proteoglycans Pathway (833812); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); PI3K-Akt Signaling Pathway (692234)

This gene encodes a member of the tenascin family of extracellular matrix glycoproteins. The tenascins have anti-adhesive effects, as opposed to fibronectin which is adhesive. This protein is thought to function in matrix maturation during wound healing, and its deficiency has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. It is one of four genes in this cluster which have been duplicated. The duplicated copy of this gene is incomplete and is a pseudogene which is transcribed but does not encode a protein. The structure of this gene is unusual in that it overlaps the CREBL1 and CYP21A2 genes at its 5' and 3' ends, respectively. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

TNXB: Appears to mediate interactions between cells and the extracellular matrix. Substrate-adhesion molecule that appears to inhibit cell migration. Accelerates collagen fibril formation. May play a role in supporting the growth of epithelial tumors. Defects in TNXB are the cause of tenascin-X deficiency (TNXD). TNXD leads to an Ehlers-Danlos-like syndrome characterized by hyperextensible skin, hypermobile joints, and tissue fragility. Tenascin-X-deficient patients, however, lack atrophic scars, a major diagnostic criteria for classic Ehlers- Danlos. Delayed wound healing, which is also common in classic EDS, is only present in a subset of patients. Belongs to the tenascin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; Extracellular matrix. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; fibrillar collagen; intracellular. Molecular Function: collagen binding; heparin binding; integrin binding. Biological Process: elastic fiber assembly; cell-cell adhesion; collagen fibril organization; triacylglycerol metabolic process; collagen metabolic process; cell-matrix adhesion; regulation of JNK activity; fibril organization and biogenesis; fatty acid metabolic process; cell adhesion; actin cytoskeleton organization and biogenesis. Disease: Ehlers-danlos-like Syndrome Due To Tenascin-x Deficiency; Vesicoureteral Reflux 8

48-Strip-Wells

410 USD

96-Strip-Wells

490