Mouse CNTF (Ciliary Neurotrophic Factor) ELISA Kit | MyBioSource MBS2508068 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse CNTF (Ciliary Neurotrophic Factor) ELISA Kit

catalog :

MBS2508068

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2508068

products type :

ELISA Kit

products full name :

Mouse CNTF (Ciliary Neurotrophic Factor) ELISA Kit

products short name :

[CNTF]

other names :

[ciliary neurotrophic factor; Ciliary neurotrophic factor; ciliary neurotrophic factor; ciliary neurotrophic factor]

products gene name :

[CNTF]

other gene names :

[CNTF; CNTF; HCNTF; CNTF]

uniprot entry name :

CNTF_HUMAN

reactivity :

Mouse

sequence length :

200

specificity :

This kit recognizes Monkey NEFL in samples. No Significant cross-reactivity or interference between Monkey NEFL and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000 pg/mL. Sensitivity: 18.75 pg/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Monkey NEFL were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Monkey NEFL were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the invitro quantitative determination of Monkey NEFL concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey NEFL. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey NEFL and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey NEFL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/-2 nm. The OD value is proportional to the concentration of Monkey NEFL.You can calculate the concentration of Monkey NEFL in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

4758020

ncbi acc num :

NP_000605.1

ncbi gb acc num :

NM_000614.3

uniprot acc num :

P26441

ncbi mol weight :

22,931 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Delta-Notch Signaling Pathway (198879); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488)

ncbi summary :

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010]

uniprot summary :

CNTF: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. Belongs to the CNTF family. Chromosomal Location of Human Ortholog: 11q12.2. Cellular Component: extracellular space; cytoplasm. Molecular Function: interleukin-6 receptor binding; growth factor activity; ciliary neurotrophic factor receptor binding. Biological Process: negative regulation of photoreceptor cell differentiation; positive regulation of cell proliferation; regulation of retinal cell programmed cell death; neuron development; negative regulation of neuron apoptosis; signal transduction; muscle morphogenesis; positive regulation of tyrosine phosphorylation of Stat3 protein; growth

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590