catalog number :
MBS2508034
products type :
ELISA Kit
products full name :
Human IgG3 (Immunoglobulin G3) ELISA Kit
products short name :
[IgG3]
other names :
[immunoglobulin heavy chain (IgG3), partial]
products gene name :
[IgG3]
specificity :
This kit recognizes natural and recombinantHumanIgG3. No significant cross-reactivity or interference between HumanIgG3 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
products description :
Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanIgG3concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IgG3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IgG3and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IgG3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IgG3.You can calculate the concentration of IgG3in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
CAA09186.1
size5 :
10x96-Strip-Wells