ELISA/assay

Sheep BALP (Bone Alkaline Phosphatase) ELISA Kit

MBS2508019

24-Strip-Wells

255 USD

ELISA Kit

Sheep BALP (Bone Alkaline Phosphatase) ELISA Kit

[BALP]

[BALP]

Sheep

This kit recognizes natural and recombinant Sheep BALP. No significant cross-reactivity or interference between Sheep BALP and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 39.063-2500pg/mL. Sensitivity: Min: 23.438pg/mL; Max: 2500pg/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep BALP concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BALP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BALP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BALP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of BALP. You can calculate the concentration of BALP in the samples by comparing the OD of the samples to the standard curve.

24-Strip-Wells

255 USD

48-Strip-Wells

490

96-Strip-Wells

585

5x96-Strip-Wells

2475

10x96-Strip-Wells

4360