catalog number :
MBS2507988
products type :
ELISA Kit
products full name :
Mouse FSH (follicle-stimulating hormone) ELISA Kit
products short name :
FSH
other names :
FSH; Bromodomain-containing protein 2; O27.1.1; Really interesting new gene 3 protein
other gene names :
BRD2; KIAA9001; RING3
uniprot entry name :
BRD2_HUMAN
specificity :
This kit recognizes natural and recombinantMouseFSH. No significant cross-reactivity or interference between MouseFSH and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Quantitative Sandwich. Sensitivity: 0.938ng/mL
products description :
Intended Uses: This ELISA kit applies to the invitro quantitative determination of MouseFSHconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FSH. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FSHand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of FSH.You can calculate the concentration of FSHin the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
CAA43996.1
ncbi mol weight :
74,881 Da
uniprot summary :
BRD2: an atypical protein kinase with two bromodomains that bind to acetylated lysine residues. Specifically interacts with transcriptionally active chromatin. Elevated protein kinase activity in leukemias. Transgenic mice overexpressing BRD2 in the lymphoid system develop diffuse large-cell lymphoma. Interacts with E2F1 and with histone H4 acetylated at Lys-13. May play a role in spermatogenesis or folliculogenesis. Genetic evidence links the BRD2 gene to both juvenile myoclonic epilepsy and photoparoxysomal responses. Two isoforms of the human protein are produced by alternative splicing. Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; Kinase, protein; ATYPICAL group; BRD family. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: cytoplasm; nucleus. Molecular Function: protein binding; chromatin binding. Biological Process: regulation of transcription from RNA polymerase II promoter; nucleosome assembly; transcription, DNA-dependent; spermatogenesis; chromatin modification