catalog number :
MBS2507946
products type :
ELISA Kit
products full name :
Human THP (Tamm-Horsfall Glycoprotein) ELISA Kit
products short name :
[THP]
other names :
[Tamm-Horsfall protein; Uromodulin; Tamm-Horsfall urinary glycoprotein; THP]
products gene name :
[THP]
other gene names :
[Umod; THP]
uniprot entry name :
UROM_RAT
specificity :
This kit recognizes Human TGM3 in samples. No significant cross-reactivity or interference between Human TGM3 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.31-20ng/mL. Sensitivity: 0.19ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TGM3 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TGM3 were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human TGM3 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TGM3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TGM3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TGM3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human TGM3. You can calculate the concentration of Human TGM3 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAB33313.1
ncbi mol weight :
71,062 Da
uniprot summary :
Uromodulin: Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability. May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelia.
size5 :
10x96-Strip-Wells