Rabbit PLAU/uPA(Urokinase-Type Plasminogen Activator) ELISA Kit | MyBioSource MBS2507914 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rabbit PLAU/uPA(Urokinase-Type Plasminogen Activator) ELISA Kit

catalog :

MBS2507914

quantity :

24-Strip-Wells

price :

235 USD

product information

catalog number :

MBS2507914

products type :

ELISA Kit

products full name :

Rabbit PLAU/uPA(Urokinase-Type Plasminogen Activator) ELISA Kit

products short name :

[PLAU/uPA]

other names :

[urokinase-type plasminogen activator isoform X1; Urokinase-type plasminogen activator; urokinase-type plasminogen activator; U-plasminogen activator; plasminogen activator, urinary; plasminogen activator, urokinase]

products gene name :

[PLAU/uPA]

other gene names :

[PLAU; PLAU; ATF; QPD; UPA; URK; u-PA; BDPLT5; U-plasminogen activator; uPA]

uniprot entry name :

UROK_HUMAN

reactivity :

Rabbit

sequence length :

375

specificity :

This kit recognizes natural and recombinant Rabbit PLAU/uPA. No significant cross-reactivity or interference between Rabbit PLAU/uPA and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit PLAU/uPA concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PLAU/uPA. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PLAU/uPA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PLAU/uPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PLAU/uPA. You can calculate the concentration of PLAU/uPA in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

578819560

ncbi acc num :

XP_006717956.1

ncbi gb acc num :

XM_006717893.1

uniprot acc num :

P00749

ncbi mol weight :

46,908 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); DNA Damage Response (only ATM Dependent) Pathway (198827); Dissolution Of Fibrin Clot Pathway (106061); E2F Transcription Factor Network Pathway (137934); Endochondral Ossification Pathway (198812); FGF Signaling Pathway (137989)

ncbi summary :

This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

uniprot summary :

uPA: Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. Defects in PLAU are the cause of Quebec platelet disorder (QPD). QPD is an autosomal dominant bleeding disorder due to a gain-of-function defect in fibrinolysis. Although affected individuals do not exhibit systemic fibrinolysis, they show delayed onset bleeding after challenge, such as surgery. The hallmark of the disorder is markedly increased PLAU levels within platelets, which causes intraplatelet plasmin generation and secondary degradation of alpha-granule proteins. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; EC 3.4.21.73; Protease. Chromosomal Location of Human Ortholog: 10q22.2. Cellular Component: extracellular space; focal adhesion; cell surface; plasma membrane; extracellular region. Molecular Function: protein binding; serine-type endopeptidase activity. Biological Process: fibrinolysis; regulation of cell adhesion mediated by integrin; regulation of smooth muscle cell migration; smooth muscle cell migration; response to hypoxia; regulation of receptor activity; chemotaxis; blood coagulation; signal transduction; proteolysis; regulation of cell proliferation. Disease: Quebec Platelet Disorder; Alzheimer Disease

size1 :

24-Strip-Wells

price1 :

235 USD

size2 :

48-Strip-Wells

price2 :

450

size3 :

96-Strip-Wells

price3 :

535

size4 :

5x96-Strip-Wells

price4 :

2255

size5 :

10x96-Strip-Wells

price5 :

3975