catalog number :
MBS2507871
products type :
ELISA Kit
products full name :
Rabbit TAT (Thrombin/Antithrombin Complex) ELISA Kit
products short name :
TAT
other names :
Tat; p14; Tat
specificity :
This assay has high sensitivity and excellent specificity for detection of Rabbit TAT. No significant cross-reactivity or interference between Rabbit TAT and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rabbit TAT and all the analogues, therefore, cross reaction may still exist.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25--2000pg/mL. Sensitivity: 18.75pg/mL
products description :
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TAT in Rabbit serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit TAT. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rabbit TAT and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit TAT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rabbit TAT. You can calculate the concentration of Rabbit TAT in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_057853.1
ncbi gb acc num :
NC_001802.1
ncbi pathways :
Disease Pathway (530764); Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway (106004); HIV Infection Pathway (105983); HIV Life Cycle Pathway (105984); HIV Transcription Elongation Pathway (106001); Host Interactions Of HIV Factors Pathway (106010); Interactions Of Tat With Host Cellular Proteins Pathway (106018); Late Phase Of HIV Life Cycle Pathway (105995); Pausing And Recovery Of Tat-mediated HIV Elongation Pathway (161055); Signaling Events Mediated By HDAC Class III Pathway (137972)