Porcine IFN-gamma (Interferon Gamma) ELISA Kit | MyBioSource MBS2507844 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine IFN-gamma (Interferon Gamma) ELISA Kit

catalog :

MBS2507844

quantity :

24-Strip-Wells

price :

210 USD

product information

catalog number :

MBS2507844

products type :

ELISA Kit

products full name :

Porcine IFN-gamma (Interferon Gamma) ELISA Kit

products short name :

[IFN-gamma]

other names :

[interferon gamma; Interferon gamma; interferon gamma; IFN-gamma; immune interferon; interferon, gamma; Immune interferon]

products gene name :

[IFN-gamma]

other gene names :

[IFNG; IFNG; IFG; IFI; IFN-gamma]

uniprot entry name :

IFNG_HUMAN

reactivity :

Porcine

sequence length :

166

specificity :

This kit recognizes Porcine IFN-beta in samples. No significant cross-reactivity or interference between Porcine IFN-beta and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Porcine IFN-beta were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Porcine IFN-beta were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine IFN-beta concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine IFN-beta. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine IFN-beta and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine IFN-beta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Porcine IFN-beta. You can calculate the concentration of Porcine IFN-beta in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

56786138

ncbi acc num :

NP_000610.2

ncbi gb acc num :

NM_000619.2

uniprot acc num :

P01579

ncbi mol weight :

19,348 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485)

ncbi summary :

This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]

uniprot summary :

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family. Protein type: Secreted; Secreted, signal peptide; Cytokine; Membrane protein, integral. Chromosomal Location of Human Ortholog: 12q14. Cellular Component: extracellular space; extracellular region; external side of plasma membrane. Molecular Function: interferon-gamma receptor binding; cytokine activity. Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; positive regulation of interleukin-23 production; negative regulation of smooth muscle cell proliferation; apoptosis; positive regulation of interleukin-12 production; positive regulation of osteoclast differentiation; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of killing of cells of another organism; sensory perception of mechanical stimulus; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-1 beta secretion; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; neutrophil chemotaxis; positive regulation of synaptic transmission, cholinergic; adaptive immune response; negative regulation of myelination; CD8-positive, alpha-beta T cell differentiation during immune response; unfolded protein response; response to virus; cytokine and chemokine mediated signaling pathway; defense response to protozoan; positive regulation of tumor necrosis factor production; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of interleukin-12 biosynthetic process; negative regulation of interleukin-17 production; positive regulation of chemokine biosynthetic process; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; cell motility; positive regulation of neuron differentiation; regulation of insulin secretion. Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Mycobacterium Tuberculosis, Susceptibility To; Tuberous Sclerosis 2; Human Immunodeficiency Virus Type 1, Susceptibility To

size1 :

24-Strip-Wells

price1 :

210 USD

size2 :

48-Strip-Wells

price2 :

400

size3 :

96-Strip-Wells

price3 :

475

size4 :

5x96-Strip-Wells

price4 :

1975

size5 :

10x96-Strip-Wells

price5 :

3480