Human FASN(Fatty Acid Synthase)ELISA Kit | MyBioSource MBS2507799 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human FASN(Fatty Acid Synthase)ELISA Kit

catalog :

MBS2507799

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2507799

products type :

ELISA Kit

products full name :

Human FASN(Fatty Acid Synthase)ELISA Kit

products short name :

FASN

other names :

fatty acid synthase; Fatty acid synthase; fatty acid synthase; short chain dehydrogenase/reductase family 27X, member 1; fatty acid synthase

products gene name :

FASN

other gene names :

FASN; FASN; FAS; OA-519; SDR27X1; FAS

uniprot entry name :

FAS_HUMAN

reactivity :

Human

sequence length :

2511

specificity :

This kit recognizes natural and recombinant Human FASN. No significant cross-reactivity or interference between Human FASN and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: 0.188ng/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human FASN concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FASN. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FASN and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FASN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of FASN. You can calculate the concentration of FASN in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

41872631

ncbi acc num :

NP_004095.4

ncbi gb acc num :

NM_004104.4

uniprot acc num :

P49327

ncbi mol weight :

273,427 Da

ncbi pathways :

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Activation Of Gene Expression By SREBF (SREBP) Pathway (685552); ChREBP Activates Metabolic Gene Expression Pathway (106104); Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004)

ncbi summary :

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]

uniprot summary :

FASN: a multifunctional enzyme. Catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. Protein type: Lipid Metabolism - fatty acid biosynthesis; Hydrolase; EC 2.3.1.85; EC 1.3.1.39; EC 1.1.1.100; EC 2.3.1.41; Acetyltransferase; EC 4.2.1.59; EC 3.1.2.14; Lyase; EC 2.3.1.38; EC 2.3.1.39; Oxidoreductase. Chromosomal Location of Human Ortholog: 17q25. Cellular Component: Golgi apparatus; glycogen granule; mitochondrion; membrane; cytoplasm; melanosome; plasma membrane; cytosol. Molecular Function: protein homodimerization activity; fatty-acid synthase activity; 3-oxoacyl-[acyl-carrier-protein] synthase activity; myristoyl-[acyl-carrier-protein] hydrolase activity; zinc ion binding; enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity; enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity; drug binding; [acyl-carrier-protein] S-acetyltransferase activity; 3-oxoacyl-[acyl-carrier-protein] reductase activity; protein binding; 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; oleoyl-[acyl-carrier-protein] hydrolase activity; palmitoyl-[acyl-carrier-protein] hydrolase activity; [acyl-carrier-protein] S-malonyltransferase activity; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity. Biological Process: osteoblast differentiation; vitamin metabolic process; acetyl-CoA metabolic process; pantothenate metabolic process; energy reserve metabolic process; positive regulation of cellular metabolic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; fatty acid metabolic process; water-soluble vitamin metabolic process; fatty acid biosynthetic process

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage