catalog number :
MBS2507735
products type :
ELISA Kit
products full name :
Human SDF-1 (Stromal Cell Derived Factor 1) ELISA Kit
products short name :
[SDF-1]
other names :
[stromal cell-derived factor 1 isoform alpha; Stromal cell-derived factor 1; stromal cell-derived factor 1; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); stromal-derived factor 1; xSDF-1; chemokine (C-X-C motif) ligand 12<; C-X-C motif chemokine 12]
products gene name :
[SDF-1]
other gene names :
[cxcl12; cxcl12; sdf1; sdf-1; xSDF-1; xSDF-1alpha; xSDF-1]
uniprot entry name :
SDF1_XENLA
specificity :
This kit recognizes natural and some recombinant Human SDF-1 . No significant crossreactivity or interference between Human SDF-1 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
products description :
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human SDF-1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human SDF-1 . Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human SDF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human SDF-1 , biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SDF-1 . The concentration of Human SDF-1 in samples can be calculated by comparing the OD of the samples with the standard curve.
ncbi acc num :
NP_001165249.1
ncbi gb acc num :
NM_001171778.1
ncbi mol weight :
10,144 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (119282); Cytokine-cytokine Receptor Interaction Pathway (460); Intestinal Immune Network For IgA Production Pathway (128758); Intestinal Immune Network For IgA Production Pathway (128670)
uniprot summary :
Chemoattractant. Activates the C-X-C chemokine receptor cxcr4 to induce a rapid and transient rise in the level of intracellular calcium ions, and chemotaxis. Signaling with cxcr4 mediates the directional movement of mesodermal cells during gastrulation.
size5 :
10x96-Strip-Wells