catalog number :
MBS2507721
products type :
ELISA Kit
products full name :
Mouse GATA3 (GATA Binding Protein 3) ELISA Kit
products short name :
[GATA3]
other names :
[GATA-binding factor 3; GATA-binding factor 3; GATA-binding factor 3; GATA-3; NF-E1; transcription factor NF-E1c; GATA binding protein 3; Transcription factor NF-E1c]
products gene name :
[GATA3]
other gene names :
[GATA3; GATA3; GATA-3]
uniprot entry name :
GATA3_CHICK
specificity :
This kit recognizes Mouse fT4 in samples. No significant cross-reactivity or interference between MousefT4 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level MousefT4 were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level MousefT4 were testedon 3 different plates, 20 replicates in eachplate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse fT4 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse fT4. During the reaction, Mouse fT4 in the sample or standard competes with a fixed amount of Mouse fT4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to MousefT4. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Mouse fT4 in the samples is then determined by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001008444.1
ncbi gb acc num :
NM_001008444.1
ncbi mol weight :
48,197 Da
uniprot summary :
Transcriptional activator which probably serves as a general switch factor for cell-specific development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of genes.
size5 :
10x96-Strip-Wells